Abstracts: Our laboratory have developed a novel RNA interference (RNAi) screening system using original library and the web-based bioinformatics tool. This aims to synthesize and discover novel candidate of molecular medicine. Using the system we have succeeded to find a novel short hairpin RNA (shRNA) which led to cancer cell apoptosis. We were interested in the results because the several base pair of RNA regulated cancer cell fate. The finding has impact on the development of new medicine. For example, a double strand artificial RNA consist of the shRNA sequence could be the candidate of anti-cancer molecular medicine. In addition, the target gene product could be a new target of conventional low molecular weight chemical compounds. For further investigation, we incorporated RNAi technology for planarian, an invertebrate animal model. Planarian has many genes which are common to human. Relatively easy cultivation of planarian facilitates the use for the safety test. Through our knockdown biotechnology, you can perform and experience the development of nucleic acid medicine from the synthesis of shRNA to the evaluation using cells and animals.
The cause of the sickness is often caused by the abnormal activity of cells. For example, tumour is often due to the uncontrolled proliferation of cancer cells. If you can control the function of genes that affect the cancer cell activity, there is a possibility that the tumor may be extinguished.
Our laboratory are developing technologies utilizing nucleic acids to control the function of genes. Our goal is to develop an effective nucleic acid medicine for diseases. Specifically, the basic technique based on the RNAi method (synthesis of nucleic acid library, genetic recombination, cancer cell culture, characterization of raw cells by fluorescent labeling, high efficiency assay by flow cytometer, The creation of nucleic acids in a useful artificial sequence through the target gene Web search system SPICE and the like nucleotide sequence analysis. At the same time, we aim to find a useful target gene and show the role of the gene. Furthermore, by using the reproduction model animal Planaria, the effect of RNA interference is evaluated by an individual.
Another study is the genome analysis of the Flexivirga Alba ST13T, a new genus and a species of actinomycetes discovered by our laboratory. The objective is to elucidate the genes involved in the detoxification of hexavalent chromium, which is designated as an environmental pollutant.